Composite

Part:BBa_K1253112:Design

Designed by: Rohan Sinha   Group: iGEM14_TAS_Taipei   (2014-06-27)


LacI self-regulatory oscillator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The TetR/RFP and GFP allows for physical observation of the oscillatory peaks and troughs. IPTG can be used to tune the oscillator. Promoter strength differences and repressor half-life differences do not dampen oscillation.


Source

This part was made using the BioBrick parts from the 2013 Spring Distribution kit.

References