Composite
Part:BBa_K1253112:Design
Designed by: Rohan Sinha Group: iGEM14_TAS_Taipei (2014-06-27)
LacI self-regulatory oscillator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The TetR/RFP and GFP allows for physical observation of the oscillatory peaks and troughs. IPTG can be used to tune the oscillator. Promoter strength differences and repressor half-life differences do not dampen oscillation.
Source
This part was made using the BioBrick parts from the 2013 Spring Distribution kit.